PROTEOLYTIC POTENTIAL OF PSEUDOMONAS FLUORESCENS ISOLATED FROM REFRIGERATED RAW MILK

Autores

  • Cláudia Lúcia Oliveira Pinto1 EPAMIG
  • Solimar Gonçalves Machado Federal University of Viçosa, Viçosa
  • Rodrigo Rezende Cardoso Federal University of Viçosa
  • Rita Maria Alves Brazilian Agricultural Research Corporation's, Embrapa Wheat, Passo Fundo, RS, Brazil
  • Maria Cristina Dantas Vanetti Federal University of Viçosa, Viçosa

DOI:

https://doi.org/10.21206/rbas.v4i2.254

Resumo

The growth rate and the proteolytic activity of Pseudomonas fluorescens strains 07A and 041, isolated from cow’s milk, were evaluated at 2, 4, 7 and 10ºC. P. fluorescens promoted protein degradation during storage of milk samples as observed by Proteolytic activity measurement, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and heat stability of milk. Casein hydrolysis resulted in loss of thermal stability of milk and in formation of fragments of low and medium molecular mass. Temperatures up to 10°C did not
guarantee raw milk quality when contamination by P. fluorescens was equal or higher than 106 cfu/mL.

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Biografia do Autor

Cláudia Lúcia Oliveira Pinto1, EPAMIG

Agriculture and Livestock Research Corporation's of Minas Gerais, EPAMIG, Viçosa, MG, Brasil. 

Solimar Gonçalves Machado, Federal University of Viçosa, Viçosa

Department of Microbiology, Federal University of Viçosa, Viçosa, 36570-000, MG, Brazil.

Rodrigo Rezende Cardoso, Federal University of Viçosa

Department of Food Science and Technology, Federal University of Viçosa, Viçosa, 36570-000, MG, Brazil.

Rita Maria Alves, Brazilian Agricultural Research Corporation's, Embrapa Wheat, Passo Fundo, RS, Brazil

Brazilian Agricultural Research Corporation's, Embrapa Wheat, Passo Fundo, RS, Brazil

Maria Cristina Dantas Vanetti, Federal University of Viçosa, Viçosa

Department of Microbiology, Federal University of Viçosa, Viçosa, 36570-000, MG, Brazil.

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Publicado

2014-12-30

Como Citar

Pinto1, C. L. O., Machado, S. G., Cardoso, R. R., Alves, R. M., & Vanetti, M. C. D. (2014). PROTEOLYTIC POTENTIAL OF PSEUDOMONAS FLUORESCENS ISOLATED FROM REFRIGERATED RAW MILK. Revista Brasileira De Agropecuária Sustentável, 4(2). https://doi.org/10.21206/rbas.v4i2.254

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