Reuse of expired dairy products and sewage in the production of biogas by anaerobic digestion




Expired dairy products, Anaerobic digestion, Reverse logistics, Methane, biogas


Dairy industry is a prominent sector of the agro-industry with global production of 906 million tonnes of milk. Several dairy products are wasted due their high perishability. Anaerobic digestion is a sustainable alternative for reducing this waste aiming biogas production. This study evaluated the co-digestion of expired dairy products (EDP) with synthetic sewage from two assays using two types of dairy waste, separately: Assay I (EDP mixture of 80% of milk + 15% of yogurt + 5% of milk cream) and Assay II (industrial raw mixture of EDP). Both assays were composed of 20 g COD L-1 (Chemical Oxygen Demand per Liter) using anaerobic batch reactors (1.0 L) assembled in duplicates with 0.5 L headspace (N2) and working volume of 0.5 L with initial pH 8.2, at 37 °C, in a static mode. The cumulative CH4 productions were 2722.5 NmL in 31 days of operation (Assay I) and 3140.0 NmL in 25 days of operation (Assay II). Equivalent CH4 yields were obtained for the both assays with ~330 NmLCH4 g CODrem-1. Carbohydrates and COD removals were 96.8% and 98.0%; 87.3% and 89.4%, respectively. The co-digestion of EDP with sewage was effective to CH4 production with efficient organic matter removal. These results encourage new strategies for reuse of expired dairy products by the use of anaerobic digestion.


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ADAMES, L. V.; PIRES, L. O.; ADORNO, M. A. T.; MAINTINGUER, S. I. Hydrogen production in anaerobic continuous flow reactor using crude glycerol from biodiesel production. Revista Materia, v. 26, n. 2, 2021.

ADGHIM, M.; Abdallah M.; SAAD, S.; Shanableh, A.; Sartaj, M.; El Mansouri, A. E. Comparative life cycle assessment of anaerobic co-digestion for dairy waste management in large-scale farms. Journal of Cleaner Production, v. 256, p. 120320, 2020.

AHMAD, T.; Aadil, R. M.; Ahmed, H.; Rahman, U.; Soares, B. C. V.; Souza, S. L. Q.; Pimentel, T. C.; Scudino, H.; Guimarães, J. T.; Esmerino, E. A.; FreitaS, M. Q.; ALMADA R. B.; VENDRAMEL, S. M. R.; SILVA, M. C., CRUZ, A. G. Treatment and utilization of dairy industrial waste: A review. Trends in Food Science and Technology, v. 88, n. April, p. 361-372, 2019.

ANA. Agência Nacional de Águas (ANA) - Atlas Esgotos - Despoluição de bacias hidrográficas. Brasília, DF: Agência Nacional de Águas, Secretaria Nacional de Saneamento Ambiental, 2017.

ANGELIDAKI, I.; ALVES, M. BOLZONELLA, D.; BORZACCONI, L.; CAMPOS, J. L.; GUWY, A. J.; KALYUZHNYI, S.; JENICEK, P.; LIER, J. B. V. Defining the biomethane potential (BMP) of solid organic wastes and energy crops?: a proposed protocol for batch assays. Water Science & Technology—WST, p. 927-934, 2009.

APHA, W. E. F. AWWA, 1995. Standard Methods for the Examination of Water and Wastewater. Amer. Pub. Health Association. Washington DC, 1998.

CNA. Comunicado Técnico: Pesquisa Pecuária Municipal 2020Confederação da Agricultura e Pecuária do Brasil. Brasília - DF: [s.n.]. Disponível em: . Access in: 27 jan. 2022.

DUBOIS, M.; GILLES, K. A.; HAMILTON, J. K.; REBERS, P.A.; SMITH, F. Colorimetric Method for Determination of Sugars and Related Substances. Analytical Chemistry, v. 28, n. 3, p. 350-356, 1956.

FAO. Dairy Market Review: Overview of global dairy market developments in 2020. Rome: [s.n.]. Disponível em: <>. Access in: 7 dez. 2021.

HANAKI, K.; MATSUO, T.; NAGASE, M. Mechanism of Inhibition Caused by Long-Chain Fatty Acids in Anaerobic Digestion Process. 1981.

HASSAN, A. N.; NELSON, B. K. Invited review: Anaerobic fermentation of dairy food wastewaterJournal of Dairy Science, nov. 2012.

HERBERT, D.; PHILIPPS, O.S.; STRANG, R. E. Carbohydrate analysis. Methods Enzymol, v. 5B, n. 2, p. 265-277, 1971.

KOPSAHELIS, A.; Stavropoulos, K.; ZAFIRI, C.; KORNAROS, M. Anaerobic co-digestion of End-of-Life dairy products with agroindustrial wastes in a mesophilic pilot-scale two-stage system: Assessment of system’s performance. Energy Conversion and Management, v. 165, n. April, p. 851-860, 2018.

LV, Y.; CHANG, N; LI, Y. Y.; LIU, J. Anaerobic co-digestion of food waste with municipal solid waste leachate: A review and prospective application with more benefits. Resources, Conservation and Recycling. Elsevier B.V., 2021.

MAINTINGUER, S. I.; FERNANDES, B. S.; DUARTE, I. C. S.; SAAVEDRA, N. K.; ADORNO, M. A. T.; VARESCHE, M. B. Fermentative hydrogen production by microbial consortium. International Journal of Hydrogen Energy, v. 33, n. 16, p. 4309-4317, 2008.

MARTÍN-GONZÁLEZ, L.; Colturato, L. F.; FONT, X.; VICENT T. Anaerobic co-digestion of the organic fraction of municipal solid waste with FOG waste from a sewage treatment plant: Recovering a wasted methane potential and enhancing the biogas yield. Waste Management, v. 30, n. 10, p. 1854-1859, 2010.

NOBLECOURT, A.; Christophe, G.; Larroche, C.; Fontanille, P. Hydrogen production by dark fermentation from pre-fermented depackaging food wastes. Bioresource Technology, v. 247, p. 864-870, 2018.

SAGE, M.; DAUFIN, G.; GESAN-GUIZIOU, G. Effect of prehydrolysis of milk fat on its conversion to biogas. Journal of Dairy Science, v. 91, n. 10, p. 4062-4074, 2008.

SAKARIKA, M.; Stavropoulos, K.; Kopsahelis, A.; Koutra, E.; Zafiri, C.; Kornaros, M. Two-stage anaerobic digestion harnesses more energy from the co-digestion of end-of-life dairy products with agro-industrial waste compared to the single-stage process. Biochemical Engineering Journal, v. 153, n. July 2019, 2020.

SAR, T.; Harirchi, S.; Ramezani, M.; Bulkan, G.; Akbas, M. Y.; Pandey, A.; Taherzadeh, M. J. Potential utilization of dairy industries by-products and wastes through microbial processes: A critical review. Science of The Total Environment, v. 810, p. 152253, 2022.

SIDDIQUE, M. N. I.; WAHID, Z. A. Achievements and perspectives of anaerobic co-digestion: A review. Journal of Cleaner Production. Elsevier Ltd. 1, 2018.

SIVAKUMAR, M. P.; Bhagiyalakshmi, M.; Anbarasu, K. Anaerobic treatment of spoiled milk from milk processing industry for energy recovery - A laboratory to pilot scale study. Fuel. v. 96, p. 482-486, feb. 2012.

STAVROPOULOS, K. P.; Kopsahelis, A.; Zafiri, C.; Kornaros, M. Effect of pH on Continuous Biohydrogen Production from End-of-Life Dairy Products (EoL-DPs) via Dark Fermentation. Waste and Biomass Valorization, v. 7, n. 4, p. 753-764, 2016.

USMANI, Z.; SHARMA, M.; GAFFEY, J.; SHARMA, M.; DEWHURST, R.J.; MOREAU, B.; NEWBOLD, J.; CLARK, W.; THAKUR, V.K.; GUPTA, V. K. Valorization of dairy waste and by-products through microbial bioprocesses. Bioresource Technology, p. 126444, nov. 2021.

VDI 4630. Fermentation of organic materials Characterization of the substrate, sampling, collection of material data, fermentation tests. [s.l: s.n.].

VERZOLA, M. A. Destinação de Leite e Laticínios Residuários. [s.l: s.n.].

VON-SPERLING, M. Introdução à qualidade das águas e ao tratamento de esgotos. 4. ed. Belo Horizonte: UFMG, 2014.

WARE, A.; POWER, N. Modelling methane production kinetics of complex poultry slaughterhouse wastes using sigmoidal growth functions. Renewable Energy, v. 104, p. 50-59, 2017.

WU, X.; Dong, C.; Yao, W.; Zhu, J. Anaerobic digestion of dairy manure influenced by the waste milk from milking operations. Journal of Dairy Science, v. 94, n. 8, p. 3778-3786, 2011.

XU, F.; LI, Y.; GE, X; YANG, L.; LI, Y. Anaerobic digestion of food waste – challenges and opportunities. Bioresource Technology, v. 247, n. July 2017, p. 1047-1058, 2018.

ZUO, X.; YUAN, H.; WACHEMO, A. C; WANG, X; ZHANG, L.; LI, J.; WEN, H.; WANG, J.; LI, X. The relationships among sCOD, VFAs, microbial community, and biogas production during anaerobic digestion of rice straw pretreated with ammonia. Chinese Journal of Chemical Engineering, v. 28, n. 1, p. 286-292, 2020.




Como Citar

Marin, D. F. C., Mendoza, L. M., Carvalho Júnior, R. P. de, & Maintinguer, S. I. (2022). Reuse of expired dairy products and sewage in the production of biogas by anaerobic digestion. Engenharia Na Agricultura, 30(Contínua), 303–310.



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