DEVELOPMENT AND VALIDATION OF ANALYTICAL METHODOLOGY BASED ON DIGITAL IMAGES OF SPOT TEST FOR DETERMINATION OF ETHANOL IN CACHAÇA

Authors

  • Kenia Dias Pessoa Federal University of Viçosa
  • Willian Toito Suarez Federal University of Viçosa
  • Renata Pereira Lopes Federal University of Viçosa
  • Marina Ferreira dos Reis Federal University of Viçosa
  • Mathews de Oliveira Krambeck Franco Federal University of Viçosa
  • Vagner Bezerra dos Santos Federal University of Pará

DOI:

https://doi.org/10.18540/jcecvl3iss7pp0995-1010

Keywords:

cachaça, ethanol, RGB, determination

Abstract

In this work it is carried out the development, optimization and validation of analytical methodology for determination of ethanol in cachaça samples. The method was based on digital image analysis spot test performed with the analyte of interest, captured by a camera in a system built for this purpose. The principle of the method is measurement of tones obtained after the occurrence of the colorimetric reaction employing the RGB color model, color components which varies in proportion to the analyte concentration. For the determination of ethanol, it was employed the reaction of redox in which chromium (VI) is reduced to chromium (III) or chromium (II) and the ethanol is oxidized to acetaldehyde, generating change in the yellow-orange color to blue-green. By determining the ethanol content, a linear calibration curve in the range from 10 to 70% v/v ethanol was obtained, with a coefficient of determination r2 = 0.9910 and limits of detection and quantification respectively equal to 1.72 % v/v and 5.74% v/v. Comparison of results for the cachaça samples analysis using the developed method and the reference method showed no significant differences between both methods, which were analyzed using the paired t-test, 95% confidence. The analysis of six cachaça samples showed that three of them had ethanol content outside the range permitted by law. Besides the image capture system is versatile and robust and can be used in situ analysis.

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Author Biographies

Kenia Dias Pessoa, Federal University of Viçosa

Department of Chemistry/Analytical Chemsitry

Willian Toito Suarez, Federal University of Viçosa

Department of Chemistry/Analytical Chemsitry

Renata Pereira Lopes, Federal University of Viçosa

Department of Chemistry/Analytical Chemsitry

Marina Ferreira dos Reis, Federal University of Viçosa

Department of Chemistry/Analytical Chemsitry

Mathews de Oliveira Krambeck Franco, Federal University of Viçosa

Department of Chemistry/Analytical Chemsitry

Vagner Bezerra dos Santos, Federal University of Pará

Department of Chemistry/Analytical Chemsitry

Published

2017-07-19

How to Cite

Pessoa, K. D., Suarez, W. T., Lopes, R. P., dos Reis, M. F., Franco, M. de O. K., & dos Santos, V. B. (2017). DEVELOPMENT AND VALIDATION OF ANALYTICAL METHODOLOGY BASED ON DIGITAL IMAGES OF SPOT TEST FOR DETERMINATION OF ETHANOL IN CACHAÇA. The Journal of Engineering and Exact Sciences, 3(7), 0995–1010. https://doi.org/10.18540/jcecvl3iss7pp0995-1010

Issue

Section

Analytical Chemistry

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