In situ immobilization of Cal B enzyme in mesoporous materials of the MCM-48 type using different ionic solids: synthesis and application in esterification reactions

Catia Santin Zanchett Battiston; Carolina Elisa Demaman Oro; Aline M. Moreira Ficanha; Paloma Truccolo Reato; Natalia Paroul; Rogério Marcos Dallago; Marcelo Luis Mignoni

doi:10.18540/jcecvl10iss3pp18614

Authors

Catia Santin Zanchett Battiston Instituto Federal de Educação, Ciência e Tecnologia do Rio Grande do Sul, Brazil https://orcid.org/0000-0001-7418-5018
Carolina Elisa Demaman Oro Universidade Regional Integrada do Alto Uruguai e das Missões, Brazil
Aline M. Moreira Ficanha Universidade Regional Integrada do Alto Uruguai e das Missões, Brazil
Paloma Truccolo Reato Universidade Regional Integrada do Alto Uruguai e das Missões, Brazil https://orcid.org/0000-0003-4038-2034
Natalia Paroul Universidade Regional Integrada do Alto Uruguai e das Missões, Brazil https://orcid.org/0000-0001-9809-7944
Rogério Marcos Dallago Universidade Regional Integrada do Alto Uruguai e das Missões, Brazil
Marcelo Luis Mignoni Universidade Regional Integrada do Alto Uruguai e das Missões, Brazil https://orcid.org/0000-0003-4241-7747

DOI:

https://doi.org/10.18540/jcecvl10iss3pp18614

Keywords:

Synthesis of geranyl, Ionic Solids, Enzymatic route catalyst, Industrial applications

Abstract

The ionic solids [C₁₆MI]Cl and [C₁₄MI]Cl were used as structure directors on MCM-48 mesoporous support with immobilized CALB lipase for the synthesis of esters from geraniol. Significant results were obtained in the synthesis of geranyl acetate and geranyl butyrate. Enzyme concentration, temperature and molar ratio of substrates were parameters that affect the synthesis yield. The highest temperature (60°C) and highest enzyme concentration (13%) conditions showed the best ester conversion results. For both esters, the ionic liquid [C₁₆MI]Cl showed better conversion results and reached a value above 38% of conversion. Furthermore, the support tested in the present study for application in ester synthesis is cost-effective. In addition, the enzymatic route to obtain the esters presents mild reaction conditions and the results show potential for application in industrial applications.