STUDY OF LIGNIN-PEROXIDASE FOR REMOVAL OF 2-CHLOROPHENOL IN LIQUID EFFLUENT

Abstract

In this work, we studied the removal of 2-chlorophenol in liquid effluent with lignin-peroxidase as biocatalyst. Therefore, we proposed the enzymatic bioremediation by discontinuous process of effluent containing 20 mg/L of 2-chlorophenol, using Lignin peroxidase (LiP) concentrations between 0.012 and 0.026 mg/mL and of hydrogen peroxide (H2O2) between 10 and 50 mmol/L, at 30ºC and pH of 4.2. The samples were collected for 2, 4, 8, 12 and 24 h reaction times. Chromatographic analysis revealed 96,5% reduction of 2-chlorophenol when concentrations of 0.026 mg/mL LiP and 50 mmol/L H2O2 were used. We observed effective results only after 24 h of treatment, regardless of LiP and H2O2 concentrations. Analysis using mass spectroscopy revealed possible formations of benzaldehyde and phthalic acid, and almost all the 2-chlorophenol consumption was confirmed after 24 h of treatment in the ideal condition.