Evaluation of magnetic carriers employed for immobilization of lipase from candida rugosa

Otávio Domingues; Letícia Karen dos Santos; Rondinelli Donizetti Herculano; Danilo Luiz Flumignan; Ariela Veloso de Paula

doi:10.18540/jcecvl6iss4pp0498-0504

Authors

Otávio Domingues Universidade Estadual Paulista "Júlio de Mesquita Filho" https://orcid.org/0000-0002-4901-1769
Letícia Karen dos Santos Universidade Estadual Paulista "Júlio de Mesquita Filho" https://orcid.org/0000-0002-7329-5298
Rondinelli Donizetti Herculano Universidade Estadual Paulista "Júlio de Mesquita Filho" https://orcid.org/0000-0001-7236-0847
Danilo Luiz Flumignan Universidade Estadual Paulista "Júlio de Mesquita Filho" https://orcid.org/0000-0003-3207-4891
Ariela Veloso de Paula Universidade Estadual Paulista "Júlio de Mesquita Filho" https://orcid.org/0000-0002-2454-9749

DOI:

https://doi.org/10.18540/jcecvl6iss4pp0498-0504

Keywords:

Infrared spectroscopy, Enzyme immobilization, Covalent bonding, Maghemite, Magnetic nanoparticles

Abstract

Currently, the use of magnetic nanoparticles has aroused interest in industrial processes, and the combination of their properties with the immobilization of lipases has been developed in order to produce carriers of easy separation of the reaction medium. In this context, the objective of the present study was to immobilize Candida rugosa lipase in magnetic nanoparticles, such as magnetite and maghemite, by physical adsorption and covalent bonding. The biocatalysts were evaluated by infrared spectroscopy (FTIR) and hydrolytic activity analysis. Thus, from the analyses performed, the best biocatalyst obtained was the immobilized by covalent bond in maghemite, presenting a hydrolytic activity of 174.67 U/g.